Autoclave is a commonly used sterilization equipment. Its main working media include steam, water, compressed air and electricity. Its working principle is to thoroughly sterilize material through water vapor under high temperature and high pressure and the large amount of latent heat it releases.

Sterilization principle of high pressure steam sterilizer:

1. High temperature

The working principle of an autoclave is mainly to inactivate bacteria through high temperature. The high pressure refers to the formation of bubbles in gas or liquid under high pressure under high temperature, which will cause the internal pressure of the sterilizer to increase, and the pressure in the sterilization pot will increase due to the temperature rise. A temperature higher than 100°C is obtained, which further coagulates and denatures the bacterial proteins, thereby inactive the bacteria.

2. High pressure

High pressure can damage the structure and function of bacteria. The pressure under high pressure will destroy the cell wall of bacteria and inactivate the proteins inside, thereby achieving the sterilization effect.

3. Time

The sterilization time of an autoclave is generally longer because bacteria under high temperature and pressure require a certain amount of time to be completely eliminated.

To sum up, the sterilization principle of an autoclave sterilizer is to destroy the structure and function of bacteria through a high-temperature and high-pressure environment and a long sterilization time, thereby achieving the sterilization effect.