Autoclave or high-pressure steam sterilizer is a device that uses saturated pressure steam to quickly and reliably disinfect and sterilize items, which is suitable for medical and health, scientific research, agriculture and other units. Autoclaves or high-pressure steam sterilizers are divided into portable high-pressure steam sterilizers, vertical high-pressure steam sterilizers, horizontal high-pressure steam sterilizers, etc. according to their style and size. It can sterilize medical equipment, dressings, glassware, solution culture medium, etc.  
 
Autoclave or high-pressure steam sterilizer is composed of a sterilization room, a control system, a protection device (water level protection, temperature control protection), a heating system, etc., which uses high-temperature saturated water vapor to denature the protein of microorganisms and cause the death of microorganisms within a certain period of time, so as to achieve the purpose of sterilizing moisture-resistant and heat-resistant articles.
 
When using an autoclave or high-pressure steam sterilizer, the most important thing is to exhaust the air in the pot. If there is air in the pot, the pressure pointed by the air pressure needle is not the pressure produced by saturated steam. At the same pressure, the temperature of steam mixed with air is lower than that produced by saturated steam. Therefore, only when the air in the pot is completely eliminated and the pot is full of steam, the sterilization can be complete.
 
The commonly-used method is: close the bleed valve, after power-on, when the pressure rises to 0.05MPa, open the bleed valve to release air, and then close the bleed valve after the pointer of the pressure gauge returns to zero. After closing the valve and re-energizing, when the pressure gauge rises to 0.1MPa, start timing and maintain the pressure to the specified time.